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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells
doi: 10.3390/ijms232112866
Figure Lengend Snippet: In vitro combined cytotoxic effects of βCD, CUR, and βCD-CUR. Normalized cell viability (%) after 24 h treatment against ( a ) SW480, ( b ) HCT116, and ( c ) NHDF cells. Data are shown as mean ± S.D. of three independent replicates, where statistically significant differences between CUR values versus βCD-CUR values are denoted as p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).
Article Snippet:
Techniques: In Vitro
Journal: International Journal of Molecular Sciences
Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells
doi: 10.3390/ijms232112866
Figure Lengend Snippet: The effect of CUR and βCD-CUR inclusion complex on the migration properties of CRC cell lines. ( a ) The wound healing assays for SW480 and HCT116 cells. ( b ) The bar chart represents the measurement of the distance between the boundaries of the migrating cells 24 h after the treatment. The area migrated between CUR and βCD-CUR groups was reported as mean ± S.D. from three replicates.
Article Snippet:
Techniques: Migration
Journal: International Journal of Molecular Sciences
Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells
doi: 10.3390/ijms232112866
Figure Lengend Snippet: The inhibition effects on cell invasion as assessed by transwell assay. Representative images of invaded cells stained with crystal violet on the bottom membrane of Matrigel transwell invasion insert for ( a ) SW480 cells and ( b ) HCT116 cells at 200× magnification. ( c ) Bar graphs represent the normalized average number of invaded cells per field of SW480 and HCT116 cells. Quantified data were expressed as the mean ± S.D. from three independent experiments. Statistically significant differences in comparison between CUR and βCD-CUR are denoted with a p < 0.05 (*) and p < 0.01 (**).
Article Snippet:
Techniques: Inhibition, Transwell Assay, Staining, Membrane, Comparison
Journal: International Journal of Molecular Sciences
Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells
doi: 10.3390/ijms232112866
Figure Lengend Snippet: Annexin V/FITC-PI flow cytometry analysis of SW480 and HCT116 cells treated with negative control, CUR, βCD-CUR, and camptothecin for 24 h. Cells were dual stained with Annexin V-FITC (FITC) and propidium iodide (PI). ( a ) Dot plot of SW480 with different treatments. ( b ) Dot plot of HCT116 cells with different treatments. Each set of data shown was a representative plot of three independent experiments, while percentages were the mean value of three independent experiments. Q1, necrosis; Q2, late apoptosis; Q3, early apoptosis; Q4, viable cell. ( c ) The quantification of apoptotic rate on both cell lines. Data are presented as the mean ± S.D. ( n = 3). * Significantly different, p < 0.05.
Article Snippet:
Techniques: Flow Cytometry, Negative Control, Staining
Journal: International Journal of Molecular Sciences
Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells
doi: 10.3390/ijms232112866
Figure Lengend Snippet: Induction of apoptosis through the activation of caspase-3 and subsequent cleavage of full-length PARP enzymes (116 kDa) into a large (89 kDa) subunit protein, with β-actin as a loading control for ( a ) SW480 cells and ( b ) HCT116 cells. ( c ) Cleaved PARP normalized to β-actin. Data for cleaved PARP were presented as mean ± S.D. of three independent replicates. Statistically significant changes between CUR and βCD-CUR groups are denoted as (*) with a p < 0.05.
Article Snippet:
Techniques: Activation Assay, Control