Journal: International Journal of Molecular Sciences

Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells

doi: 10.3390/ijms232112866

Figure Lengend Snippet: In vitro combined cytotoxic effects of βCD, CUR, and βCD-CUR. Normalized cell viability (%) after 24 h treatment against ( a ) SW480, ( b ) HCT116, and ( c ) NHDF cells. Data are shown as mean ± S.D. of three independent replicates, where statistically significant differences between CUR values versus βCD-CUR values are denoted as p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***).

Article Snippet: Human CRC cell lines HCT116 (ATCC ®® CCL-247TM) and SW480 (ATCC ®® CCL-228TM), as well as normal human cell control NHDF (ATCC ®® PCS-201-012TM), were cultured in the media containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 100 U/mL penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Inc., USA) and 10.0% ( v / v ) fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., USA).

Techniques: In Vitro

Journal: International Journal of Molecular Sciences

Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells

doi: 10.3390/ijms232112866

Figure Lengend Snippet: The effect of CUR and βCD-CUR inclusion complex on the migration properties of CRC cell lines. ( a ) The wound healing assays for SW480 and HCT116 cells. ( b ) The bar chart represents the measurement of the distance between the boundaries of the migrating cells 24 h after the treatment. The area migrated between CUR and βCD-CUR groups was reported as mean ± S.D. from three replicates.

Article Snippet: Human CRC cell lines HCT116 (ATCC ®® CCL-247TM) and SW480 (ATCC ®® CCL-228TM), as well as normal human cell control NHDF (ATCC ®® PCS-201-012TM), were cultured in the media containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 100 U/mL penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Inc., USA) and 10.0% ( v / v ) fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., USA).

Techniques: Migration

Journal: International Journal of Molecular Sciences

Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells

doi: 10.3390/ijms232112866

Figure Lengend Snippet: The inhibition effects on cell invasion as assessed by transwell assay. Representative images of invaded cells stained with crystal violet on the bottom membrane of Matrigel transwell invasion insert for ( a ) SW480 cells and ( b ) HCT116 cells at 200× magnification. ( c ) Bar graphs represent the normalized average number of invaded cells per field of SW480 and HCT116 cells. Quantified data were expressed as the mean ± S.D. from three independent experiments. Statistically significant differences in comparison between CUR and βCD-CUR are denoted with a p < 0.05 (*) and p < 0.01 (**).

Article Snippet: Human CRC cell lines HCT116 (ATCC ®® CCL-247TM) and SW480 (ATCC ®® CCL-228TM), as well as normal human cell control NHDF (ATCC ®® PCS-201-012TM), were cultured in the media containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 100 U/mL penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Inc., USA) and 10.0% ( v / v ) fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., USA).

Techniques: Inhibition, Transwell Assay, Staining, Membrane, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells

doi: 10.3390/ijms232112866

Figure Lengend Snippet: Annexin V/FITC-PI flow cytometry analysis of SW480 and HCT116 cells treated with negative control, CUR, βCD-CUR, and camptothecin for 24 h. Cells were dual stained with Annexin V-FITC (FITC) and propidium iodide (PI). ( a ) Dot plot of SW480 with different treatments. ( b ) Dot plot of HCT116 cells with different treatments. Each set of data shown was a representative plot of three independent experiments, while percentages were the mean value of three independent experiments. Q1, necrosis; Q2, late apoptosis; Q3, early apoptosis; Q4, viable cell. ( c ) The quantification of apoptotic rate on both cell lines. Data are presented as the mean ± S.D. ( n = 3). * Significantly different, p < 0.05.

Article Snippet: Human CRC cell lines HCT116 (ATCC ®® CCL-247TM) and SW480 (ATCC ®® CCL-228TM), as well as normal human cell control NHDF (ATCC ®® PCS-201-012TM), were cultured in the media containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 100 U/mL penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Inc., USA) and 10.0% ( v / v ) fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., USA).

Techniques: Flow Cytometry, Negative Control, Staining

Journal: International Journal of Molecular Sciences

Article Title: Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells

doi: 10.3390/ijms232112866

Figure Lengend Snippet: Induction of apoptosis through the activation of caspase-3 and subsequent cleavage of full-length PARP enzymes (116 kDa) into a large (89 kDa) subunit protein, with β-actin as a loading control for ( a ) SW480 cells and ( b ) HCT116 cells. ( c ) Cleaved PARP normalized to β-actin. Data for cleaved PARP were presented as mean ± S.D. of three independent replicates. Statistically significant changes between CUR and βCD-CUR groups are denoted as (*) with a p < 0.05.

Article Snippet: Human CRC cell lines HCT116 (ATCC ®® CCL-247TM) and SW480 (ATCC ®® CCL-228TM), as well as normal human cell control NHDF (ATCC ®® PCS-201-012TM), were cultured in the media containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific, Inc., USA) supplemented with 100 U/mL penicillin-streptomycin (Gibco, Thermo Fisher Scientific, Inc., USA) and 10.0% ( v / v ) fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., USA).

Techniques: Activation Assay, Control

Journal: PLoS ONE

Article Title: A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer

doi: 10.1371/journal.pone.0189337

Figure Lengend Snippet: (A) Immunofluorescence staining of the transmembrane luteinizing hormone receptor (LH-R) and follicle-stimulating hormone receptor (FSH-R) and the nuclear androgen receptor (AR) and estrogen receptor (ER) in HTC116 cells. (B) Negative control. BF, bright field. Scale of merged picture, 10 μM.

Article Snippet: Human CRC cell lines HTC116 (ATCC ® CCL-247 TM ) and HTB37 (ATCC HTB-37 TM ) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 100 IU/ml penicillin and 10 μg/ml streptomycin with 10% heat-inactivated, non-charcoal-stripped FBS (Sigma-Aldrich, Cat. No. F9665).

Techniques: Immunofluorescence, Staining, Negative Control

Journal: PLoS ONE

Article Title: A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer

doi: 10.1371/journal.pone.0189337

Figure Lengend Snippet: (A) Migration of HTC116 and HTB37 cells in response to FBS (10%), follicle-stimulating hormone (FSH, 10 IU/ml), luteinizing hormone (LH, 10 IU/ml), estrogen receptor (ESTR, 100 nM), progesterone (PROG, 100 nM), danazol (DAN, 80 μg/ml), or prolactin (PRL, 0.5 μg/ml). *p≤0.05, **p≤0,005. (B) Adhesion of HTC116 and HTB37 cells after treatment with FSH (10 IU/ml), LH (10 IU/ml), ESTR (100 nM), PROG (100 nM), DAN (80 μg/ml), or PRL (0.5 μg/ml) to fibronectin-coated plates. The hormones were tested in serum-free medium. Bars indicate standard deviations; *p≤0.05, **p≤0.005.

Article Snippet: Human CRC cell lines HTC116 (ATCC ® CCL-247 TM ) and HTB37 (ATCC HTB-37 TM ) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 100 IU/ml penicillin and 10 μg/ml streptomycin with 10% heat-inactivated, non-charcoal-stripped FBS (Sigma-Aldrich, Cat. No. F9665).

Techniques: Migration

Journal: PLoS ONE

Article Title: A novel potential role of pituitary gonadotropins in the pathogenesis of human colorectal cancer

doi: 10.1371/journal.pone.0189337

Figure Lengend Snippet: Effect of pituitary and gonadal glycoproteins on the proliferation of HTC116 (A, B) and HTB 37 (C, D) cells. Proliferation of HTC116 cells after treatment with (A) follicle-stimulating hormone (FSH, 1 and 10 IU/ml) or luteinizing hormone (LH, 1 and 10 IU/ml) or (B) estrogen receptor (ESTR, 100 nM), progesterone (PROG, 100 nM), danazol (DAN, 80 μg/ml), or prolactin (PRL, 0.5 μg/ml). Proliferation of HTB37 cells after treatment with (C) FSH (1 and 10 IU/ml) or LH (1 and 10 IU/ml) or (D) ESTR (100 nM), PROG (100 nM), DAN (80 μg/ml), or PRL (0.5 μg/ml).

Article Snippet: Human CRC cell lines HTC116 (ATCC ® CCL-247 TM ) and HTB37 (ATCC HTB-37 TM ) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 100 IU/ml penicillin and 10 μg/ml streptomycin with 10% heat-inactivated, non-charcoal-stripped FBS (Sigma-Aldrich, Cat. No. F9665).

Techniques: